Al-Shekhadat, R. I.; Dukhovlinov, I. V.; Kobatov, A. I.; Klimov, N. A.; Kozlov, A. P. Obtaining of the p17 recombinant protein of human immunodeficiency subtype A virus // Applied Biochemistry and Microbiology. – 2011 – № 47. – P. 730-736.
A simple and efficient method for expression in Escherichia coli cells and purification of a recombinant matrix protein, p17, of human immunodeficiency type I virus has been described. HIV-1 subtype A DNA sequence encoding p17 was obtained by amplification of the viral gag gene segment and cloned into an expression vector under the control of T7Lac promoter. The conditions for cell growth and induction of p17 synthesis by lactose and its further purification by metal chelate chromatography were optimized. p17 preparations with 97% purity were obtained; the yield of the protein of 28 mg per 1l of culture was achieved. The obtained protein was capable of binding antibodies from blood serum of a HIV-infected patient during immunoblotting.
Keywords: p17 protein, human immunodeficiency virus, HIV-1, subtype A, Gag.